selectin l ek0503 kit  (Boster Bio)

Journal: Frontiers in Immunology

Article Title: Mepolizumab treatment alters the functional phenotype of eosinophils in severe eosinophilic asthma

doi: 10.3389/fimmu.2025.1654111

Figure Lengend Snippet: Peripheral blood eosinophils from SEA patients show a different activation profile compared to HC. The percentage of CD11b + (A) , CD44 + (B) , IL-5Rα + (C) , IL-3Rα + (D) , Siglec-8 + (E) , galectin-1 + (F) , galectin-10 + (G) , and CD62L (L-selectin) + (H) eosinophils is depicted. Mann-Whitney U test; p-value is shown for each comparison.

Article Snippet: sCD62L was measured in serum samples using the human CD62L ELISA kit (R&D Systems, Minneapolis, MI, cat. no DY728) in accordance with the manufacturer’s instructions.

Techniques: Activation Assay, MANN-WHITNEY, Comparison

Journal: Frontiers in Immunology

Article Title: Mepolizumab treatment alters the functional phenotype of eosinophils in severe eosinophilic asthma

doi: 10.3389/fimmu.2025.1654111

Figure Lengend Snippet: Mepolizumab treatment modifies the functional phenotype of blood eosinophils. (A, B) Expression of CD62L (L-selectin) in healthy (HC) and SEA before (T0) and after 4 (T4), 16 (T16), and 32 (T32) weeks of mepolizumab treatment in terms of % of + cells (A) or MFI (B) ; (C) iEOS (CD62L lo ) and rEOS (CD62L hi ) eosinophils can be distinguish in peripheral blood and they are modified with mepolizumab treatment. (D) Change in the number of iEOS and rEOS with mepolizumab treatment. (E) Percentage of iEOS in healthy (HC) and SEA before (T0) and after 4 (T4), 16 (T16), and 32 (T32) weeks of mepolizumab treatment. (F) Changes in iEOS (CD62Llow) and rEOS (CD62L hi ) percentages with mepolizumab treatment. A representative donor was selected. Mann-Whitney U test to compare HC vs SEA; p-value is shown for each comparison. A mixed-effects analysis with the Geisser-Greenhouse correction followed by Holm-Šídák’s multiple comparisons test was used to address the effect of mepolizumab treatment at different time-points; *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: sCD62L was measured in serum samples using the human CD62L ELISA kit (R&D Systems, Minneapolis, MI, cat. no DY728) in accordance with the manufacturer’s instructions.

Techniques: Functional Assay, Expressing, Modification, MANN-WHITNEY, Comparison

Journal: Frontiers in Immunology

Article Title: Mepolizumab treatment alters the functional phenotype of eosinophils in severe eosinophilic asthma

doi: 10.3389/fimmu.2025.1654111

Figure Lengend Snippet: Mepolizumab treatment decreases the levels of sCD62L in serum. (A) Levels of sCD62L (sL-selectin) in serum from healthy (HC) and SEA before (T0) and after 4 (T4), 16 (T16), and 32 (T32) weeks of mepolizumab treatment; Mann-Whitney U test to compare HC vs SEA; p-value is shown for each comparison. A mixed-effects analysis with the Geisser-Greenhouse correction followed by Holm-Šídák’s multiple comparisons test was used to address the effect of mepolizumab treatment at different time-points; *p < 0.05; **p < 0.01; ***p < 0.001. (B) Heatmap plot showing the correlation of sCD62L levels in serum with the number of neutrophils, eosinophils, iEOS (CD62L lo ), and rEOS (CD62L hi ), and their CD62L surface expression (MFI). iEOS are negative for CD62L; thus, no MFI values were included. Spearman test was used for correlation. Significant p-values (< 0.05) are depicted.

Article Snippet: sCD62L was measured in serum samples using the human CD62L ELISA kit (R&D Systems, Minneapolis, MI, cat. no DY728) in accordance with the manufacturer’s instructions.

Techniques: MANN-WHITNEY, Comparison, Expressing

Journal: Frontiers in Immunology

Article Title: Mepolizumab treatment alters the functional phenotype of eosinophils in severe eosinophilic asthma

doi: 10.3389/fimmu.2025.1654111

Figure Lengend Snippet: mRNA gene expression does not always follow the same pattern as the protein surface. mRNA expression of selected targets related to (A) activation markers ( ITGAM , ITGB2 , CD44 ); (B) CD62L and its shedding ( SELL, ADAM8 ); and (C) cytokine receptors ( IL5RA, IL3RA, CSFR2A, IL1RL1, IL1RAP ) were measured by Nanostring analysis. Raw counts were normalized using top 18 stable housekeeping genes. Normalized gene expression was log 2 transformed. A paired differential expression analysis comparing T0 and T16 was performed. p-values were adjusted for multiple testing using the Benjamini-Hochberg correction. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: sCD62L was measured in serum samples using the human CD62L ELISA kit (R&D Systems, Minneapolis, MI, cat. no DY728) in accordance with the manufacturer’s instructions.

Techniques: Gene Expression, Expressing, Activation Assay, Transformation Assay, Quantitative Proteomics

Journal: Frontiers in Immunology

Article Title: Urine L-selectin reflects clinical and histological renal disease activity and treatment response in lupus nephritis across multi-ethnicity

doi: 10.3389/fimmu.2023.1200167

Figure Lengend Snippet: Comparison of uL-selectin levels among subject groups and their correlation with clinical indices among Asian (all were Chinese) (A, E, H) , non-Hispanic Caucasian (B, F) , non-Hispanic African American (C, G) and Hispanic (D) subjects. Kruskal-Wallis test and Dunn post hoc multiple comparisons test were used among subgroups across ethnicities. ROC curve analyses were performed, demonstrating the ability of uL-selectin to discriminate aLN from other subgroups (A–D) and its better performance in discriminating aLN from aNR than conventional biomarkers (C3, C4 and anti-dsDNA antibody) in the Asian (E) , Caucasian (F) and African American (G) groups. Values in the plot indicate areas under curve (AUC). uL-selectin was correlated significantly with SLEDAI, rSLEDAI in the Asian, African American, and Hispanic groups (A, C, D) , as well as with SLICC RAS in the Asian group (A, H) . With respect to the data from the African American subjects, the shown analyses have been executed after removing 2 outliers. Whereas the mean ± SD of uL-selectin level in the rest of the patients was 29 ± 31.5 (ng/mg), the mean in these 2 outliers was 3533.7 (ng/mg). Inclusion of both subjects yielded correlation coefficients of 0.54 and 0.61 with SLEDAI and rSLEDAI, respectively (not shown). aLN, active lupus nephritis; HC, healthy control; aNR, active non-renal; iLN, inactive lupus nephritis; iNR, inactive non-renal; CKD, chronic kidney disease; L-sel, urinary L-selectin adjusted by creatinine; DNA, anti-dsDNA antibody; R, Spearman’s correlation coefficient; * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Article Snippet: Urinary L-selectin (uL-selectin) was assayed using a commercially available human L-selectin enzyme-linked immunosorbent assay (ELISA) kit (DY728, R&D System; ELH-LSelectin, Raybiotech; 1:20) according to the manufacturer’s instruction. uL-selectin was normalized by urine creatinine using Creatinine Parameter Assay Kit (KGE005, R&D Systems).

Techniques: Comparison

Journal: Frontiers in Immunology

Article Title: Urine L-selectin reflects clinical and histological renal disease activity and treatment response in lupus nephritis across multi-ethnicity

doi: 10.3389/fimmu.2023.1200167

Figure Lengend Snippet: uL-selectin reflects concurrent renal pathology indices in LN. uL-selectin in LN patients correlated with AI (A) and CI (B) by Spearman correlation analysis. (C) Correlation matrix for comparison of uL-selectin and conventional metrics (serum C3, C4, anti-dsDNA and 24h proteinuria) with renal pathology AI, CI and their component attributes. The numbers in squares (upper right), and the colors and size of circles (lower left) all represent the corresponding Spearman’s correlation coefficient. The upper right and lower left halves of the plot depict the same results across an imaginary diagonal. The circles (lower left) were removed where the corresponding P value for the correlation coefficient exceeded 0.05. (D) uL-selectin could discriminate high AI (AI>6) from low AI (AI ≤ 6), and (E) could also differentiate high CI (CI>3) from low CI (CI ≤ 3), compared with conventional disease indices. Values in the plot indicate areas under curve. (F) The forest plot summarizes results from univariate and multivariate logistic regression analysis for high renal pathology CI scores. Lower urinary L-selectin was associated with an increased risk of high CI (CI>3) even after adjusting for age, gender, SLE disease duration and LN disease duration. OR values in multivariate logistic regression were adjusted OR values. AI, activity index; CI, chronicity index; R, Spearman’s correlation coefficient; L-sel/uL-selectin/Cr, urinary L-selectin adjusted by creatinine; DNA, anti-dsDNA antibody; UP, 24-hour urine protein quantity; AI-1, Endocapillary hypercellularity; AI-2, Neutrophils/karyorrhexis; AI-3, Cellular/fibrocellular crescents; AI-4, Fibrinoid necrosis; AI-5, Hyaline deposits; AI-6, Interstitial inflammation; CI-1, Glomerulosclerosis; CI-2, Fibrous crescents; CI-3, Interstitial fibrosis; CI-4, Tubular atrophy; eGFR, estimated glomerular filtration rate; OR, odds ratio; 95% CI, 95% confidence interval. * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: Urinary L-selectin (uL-selectin) was assayed using a commercially available human L-selectin enzyme-linked immunosorbent assay (ELISA) kit (DY728, R&D System; ELH-LSelectin, Raybiotech; 1:20) according to the manufacturer’s instruction. uL-selectin was normalized by urine creatinine using Creatinine Parameter Assay Kit (KGE005, R&D Systems).

Techniques: Comparison, Activity Assay, Filtration

Journal: Frontiers in Immunology

Article Title: Urine L-selectin reflects clinical and histological renal disease activity and treatment response in lupus nephritis across multi-ethnicity

doi: 10.3389/fimmu.2023.1200167

Figure Lengend Snippet: Subgroup analyses for combined AI+CI in the Chinese cohort (A–F) and US-based cohort (G–L) . uL-selectin levels (A, G) , rSLEDAI (B, H), 24h urine protein (C, I) , C3 levels (D, J) and anti-dsDNA levels (E, K) were shown in four subgroups of LN patients; Kruskal-Wallis test and Dunn’s multiple comparisons test. (F, L) ROC curve analyses were performed to discriminate HL subgroup from LH subgroup of LN patients. HH both high AI (AI>6) and high CI (CI>3); HL both high AI (AI>6) and low CI (CI ≤ 3); LH both low AI (AI ≤ 6) and high CI (CI>3); LL both low AI (AI ≤ 6) and low CI (CI ≤ 3); AI activity index; CI chronicity index; ns, no significance. * P <0.05, ** P <0.01, ****P <0.0001.

Article Snippet: Urinary L-selectin (uL-selectin) was assayed using a commercially available human L-selectin enzyme-linked immunosorbent assay (ELISA) kit (DY728, R&D System; ELH-LSelectin, Raybiotech; 1:20) according to the manufacturer’s instruction. uL-selectin was normalized by urine creatinine using Creatinine Parameter Assay Kit (KGE005, R&D Systems).

Techniques: Activity Assay

Journal: Frontiers in Immunology

Article Title: Urine L-selectin reflects clinical and histological renal disease activity and treatment response in lupus nephritis across multi-ethnicity

doi: 10.3389/fimmu.2023.1200167

Figure Lengend Snippet: Urinary L-selectin levels in active LN patients at the endpoints of follow up compared with those at baseline. (A) Urinary L-selectin levels were decreased in the complete renal remission group (CRR) (n=9, P=0.0039) at the end of follow-up, while (B) it remained stable in the partial renal remission group (PRR) (n=4, P=0.125) and (C) in the no renal remission group (NRR) (n=7, P=0.578). BL, baseline; EP, endpoint; ns, no significance. Wilcoxon matched-paired signed rank test. ** P ≤ 0.01.

Article Snippet: Urinary L-selectin (uL-selectin) was assayed using a commercially available human L-selectin enzyme-linked immunosorbent assay (ELISA) kit (DY728, R&D System; ELH-LSelectin, Raybiotech; 1:20) according to the manufacturer’s instruction. uL-selectin was normalized by urine creatinine using Creatinine Parameter Assay Kit (KGE005, R&D Systems).

Techniques:

Journal: The European respiratory journal

Article Title: Circulating Adhesion Molecules and Subclinical Interstitial Lung Disease: The Multi-Ethnic Study of Atherosclerosis

doi: 10.1183/13993003.00295-2019

Figure Lengend Snippet: Associations of circulating adhesion molecules with HAA, ILA and FVC.

Article Snippet: Serum levels of sVCAM-1 and L-Selectin were measured by ELISA (Quantikine Human Soluble VCAM Immunoassay kit, Human Soluble L-Selectin Immunoassay kit, R&D Systems) in 2,440 participants.

Techniques:

Journal: The European respiratory journal

Article Title: Circulating Adhesion Molecules and Subclinical Interstitial Lung Disease: The Multi-Ethnic Study of Atherosclerosis

doi: 10.1183/13993003.00295-2019

Figure Lengend Snippet: Associations circulating adhesion molecules with ILD events.

Article Snippet: Serum levels of sVCAM-1 and L-Selectin were measured by ELISA (Quantikine Human Soluble VCAM Immunoassay kit, Human Soluble L-Selectin Immunoassay kit, R&D Systems) in 2,440 participants.

Techniques: